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Literature summary extracted from

  • Richter, S.; Morrison, S.; Connor, T.; Su, J.; Print, C.G.; Ronimus, R.S.; McGee, S.L.; Wilson, W.R.
    Zinc finger nuclease mediated knockout of ADP-dependent glucokinase in cancer cell lines: effects on cell survival and mitochondrial oxidative metabolism (2013), PLoS ONE, 8, e65267.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.147 Homo sapiens
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.147 H-460 cell
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 Homo sapiens
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2.7.1.147 HCT-116 cell
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 Homo sapiens
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General Information

EC Number General Information Comment Organism
2.7.1.147 physiological function knockout of inc finger nucleases in each H-460 and HCT-11 cells lead to frameshift mutations in all alleles at the target site in exon 1 of ADP-dependent glucokinase ADPGK, and are ADPGK-null by immunoblotting. ADPGK knockout has little or noeffect on cell proliferation, but compromises the ability of H-460 cells to survive siRNA silencing of hexokinase-2 under oxic conditions and anoxia. No such changes are found when ADPGK is knocked out in HCT-116 cells. Knockout of ADPGK in HCT-116 cells causes few changes in global gene expression, while knockout of ADPGK in H-460 cells causes notable up-regulation of mRNAs encoding cell adhesion proteins. No consistent effect on glycolysis under oxic conditions in a variety of media is observed. Oxygen consumption rates are generally lower in the ADPGK knockouts Homo sapiens